Composition:
Casts are cylindrical structures composed mainly of mucoprotein (the Tamm-Horsefall
mucoprotein) which is secreted by epithelial cells lining the loops of Henle, the distal tubules
and the collecting ducts. The factors responsible for the precipitation of this mucoprotein are
not fully understood, but may relate to the concentration and pH of urine in these areas.
Casts may form in the presence or absence of cells in the tubular lumen. If cells (epithelial
cells, WBC) are present as a cast forms, they may adhere to, and subsequently be
surrounded by, the fibrillar protein network.
Formation: A commonly-held theory is that cellular, granular, and waxy casts represent different stages of degeneration of cells in a cast. The appearance of a cast observed in a urine sediment depends largely upon the length of time it remained in situ in the tubules prior to being shed into the urine. A cast recognizable as "cellular", for example, was shed shortly after it was formed. A waxy cast, in contrast, was retained longer in the tubular system prior to being released (see figure). General Interpretation of casts: Casts are quantified for reporting as the number seen per low power field (10x objective) and classified as to type (e.g., waxy casts, 5-10/LPF). Casts in urine from normal individuals are few or none.
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Hyaline casts | Cellular Casts |
Granular Casts | Fatty Casts |
Waxy Casts |